small airway epithelial cell growth medium  (PromoCell)

Journal: bioRxiv

Article Title: An immune-poised state of human bronchial epithelial cells mediates RSV resistance in adults

doi: 10.64898/2026.02.02.703395

Figure Lengend Snippet: a , Dot plots for the 15 most significantly enriched hallmark pathways with age in ciliated cells in donor lungs of young children (N=13) and adults (N=12) by scRNA-seq dataset analyses. The status of age association of these 15 pathways in AT1 and AT2 cells, neonatal and adult BSCs (N=9 per age group) and day 21 ALI cultures (N=3 per age group) was also evaluated. b , Heatmap of the 47 age-associated inflammatory response genes in BSCs and ALI cultures. Each lane represents one donor. c , e , Representative Western blot assays of RIG-I, p-STAT3 Y705 , and STAT3 in neonatal and adult BSCs and ALI cultures. β-Actin was loading control. Each lane is one donor. d , f , Quantification of Western blot results by densitometry normalized to β-Actin. g , h , Representative antibody staining for RIG-I (fluorescence) and p-STAT3 Y705 (chromogenic) in infant and adult lungs (N=3 donors per age). Cells residing in lung mesenchyme in the same images showed comparable levels of staining for RIG-I and p-STAT3 Y705 between the two ages. Nuclei were counter stained by Hoechst dye in g and hematoxylin in h . Data were quantified by mean fluorescence intensity (MFI) for RIG-I staining and the percentage of bronchial epithelial cells positive for p-STAT3 Y705 . Panels on the right show enlarged outlined areas in panels on the left. The yellow dotted line marks basement membrane. *p<0.05 and ns, not significant by Student’s t-test (two-tailed).

Article Snippet: BSCs in TA samples were cultured and expanded in Small Airway Epithelial Cell Growth Medium (SAGM, PromoCell, Cat# C-21070) in the presence of SMAD/ROCK/mTOR inhibitors – .

Techniques: Western Blot, Control, Staining, Fluorescence, Membrane, Two Tailed Test

Journal: bioRxiv

Article Title: An immune-poised state of human bronchial epithelial cells mediates RSV resistance in adults

doi: 10.64898/2026.02.02.703395

Figure Lengend Snippet: a , Schema of mouse flu infection and scRNA-seq in a public dataset (GSE262927). b , Dot plots of enriched hallmark pathways in BEpiC and AT1+AT2 cells at each time point after infection. Mock group is baseline control. c , Schema of mouse flu infection for lung section staining. d , f , h , J , Representative images of fluorescence staining for H3K27me3, H3K27ac and RIG-I and chromogenic staining for p-STAT3 Y705 in mock and H1N1-infected mouse lungs. Cells residing in the lung mesenchyme within the same images, which showed comparable staining between mock and IAV infection, were control for BEpiC-specific changes following infection. The bottom panels show enlarged outlined areas in the upper panels. The dotted line marks basement membrane. Nuclei were stained by Hoechst dye in fluorescence staining and by hematoxylin in chromogenic staining. Scale bars, 50 µm. e , g , i , k , Quantification by MFI or the percentage of positive epithelial cells. Bar graphs show mean ± SEM. Each dot represents one mouse. *p<0.05 and ns, not significant by one-way ANOVA followed Tukey’s multiple comparison tests.

Article Snippet: BSCs in TA samples were cultured and expanded in Small Airway Epithelial Cell Growth Medium (SAGM, PromoCell, Cat# C-21070) in the presence of SMAD/ROCK/mTOR inhibitors – .

Techniques: Infection, Control, Staining, Fluorescence, Membrane, Comparison

Journal: bioRxiv

Article Title: Type VI collagen is proportionally lower around airways and blood vessels in idiopathic pulmonary fibrosis

doi: 10.64898/2026.01.13.698558

Figure Lengend Snippet:

Article Snippet: The isolated epithelial cells were thawed from liquid nitrogen storage and maintained in Airway Epithelial Growth Medium (AEGM; PromoCell, Heidelberg, Germany) enriched with the accompanied BulletKit and 1% P/S.

Techniques: